Análise fitoquímica do extrato hidroetanólico de Turnera diffusa Willd e avaliação de seus efeitos na morte de astrócitos

Objective: To evaluate the phytochemical composition of hydroethanolic extracts from powdered aerial parts of Turnera diffusa Willd (TurneraceaeT. diffusa), as well as its toxicity in astrocytes. Methods: Chemical analyses of hydroethanolic extract from powdered aerial parts of T. diffusa were carried out using HPLC-DAD-ESI-MS/MS. In vitro assays using astrocytes culture were performed to evaluate cell death. Results: Flavone-C, O-diglycosides, such as, luteolin-8-C-[6-deoxy-2-O-rhamnosyl]-xylo-hexos-3-uloside, apigenin-8-C-[ 6-deoxy-2-O-rhamnosyl]-xylo-hexos-3-uloside and apigenin-7-O-6"-p-coumaroylglucoside were the main compounds found in this hydroethanolic extract. Concentration time-effect demonstrated the toxicity of this extract at a concentration of 1,000 mu g/mL in astrocyte culture, after 6 and 24 hours of incubation. Conclusion: In phytochemical analyses, important antioxidants (mainly flavonoids) were observed. T. diffusa extracts presented cytotoxic effect in high concentrations, leading to increased cell death in astrocyte culture.Associacao Fundo de Incentivo a Psicofarmacologia (AFIP)Centro Brasileiro de Informacoes sobre Drogas Psicotropicas (CEBRID)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Universidade Federal de São Paulo, São Paulo, SP, BrasilUniversidade Federal de São João del-Rei, Divinópolis, MG, BrasilUniversidade Federal de São Paulo, Rua Napoleao de Barros 925, BR-04024002 Sao Paulo, SP, Brazil.Web of Scienc