Evidences of metabolic alterations and cellular damages in mussels after short pulses of Ti contamination
- Monteiro, Rui
- Costa, Silvana
- Coppola, Francesca
- Freitas, Rosa
- Vale, Carlos
- Pereira, Eduarda
DOIAbstract
Mytilus galloprovincialis mussels were exposed to seawater contaminated with Ti. Initial concentrations were 4.1, 32, and 66 μg L−1 that declined during the first 24 h of the experiments, and after 48 h values were <2 μg L−1. Experiments were run in triplicate, under constant salinity and temperature. Mussels were fed every two days, and water renewed every seven days and Ti concentrations re-stabilized. During the first 28 days of experimental period, mussels were exposed to four short pulses of contamination, followed by few days of low Ti concentration between weekly contamination renewals. Then mussels were exposed to additional 14-day exposure to Ti uncontaminated seawater. Only residual Ti concentrations were measured in mussels' whol...
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