Identification and overcome of limitations of weathered oil hydrocarbons bioremediation by an adapted Bacillus sorensis strain

Because of the high production of oil and gas in Qatar, the likelihood of oil spill occurrence is most probably susceptible to happen. Contaminated soil treatment is very expensive. Cost effective mechanisms are investigated to treat this threatening issue. For that reason, bioremediation and biotechnology tools are introduced to help accelerate and remove the pollution caused by the contamination process. This paper demonstrated the importance of optimising the treatment conditions to the indigenous bacterial strain to obtain the highest biodegradation efficiency rates. The usage of biopile system technology was used with biostimulation and bioaugmentation processes. The indigenous Bacillus sonorensis (B. sonorensis) D1 bacterium played a crucial role in the biodegradation process when introduced to optimized conditions; carbon/nitrogen/phosphorus (C/N/P) (100/10/1), temperature (37 °C), surfactant tween 80 (0.12% (v/w)), and moisture (10%). Gas chromatography (mass spectrometry/flame ionization detector) (GC- (MS/FID)), Fourier transform infrared (FTIR), and colony-forming unit (CFU) analyses were performed. The diesel range organics (DRO) and polycyclic aromatic hydrocarbons (PAH) removal (%) of the weathered oil contaminated soil reached, after 160 days, 39.2% and 32.4% simultaneously when ammonium nitrate was used as a nitrogen source. Whereas urea inhibited the oil degradation process and caused the pH to rise to 9.55.The authors acknowledge the staff of the Environmental StudyCenter, Qatar University, especially Mrs Noora M. Al-Shamary and MrMazen Abu Asali, for providing support in GC analysis. We also thankthe staff of the Central Laboratories Unit, of Qatar University especiallyMr Ahmed Ali Easa and Ms Muneera Al-Qahtani for providing sup-port in the FTIR analysis. This work was supported by Qatar UniversityGrants [QUST-CAS-FALL-15/16-15:QUST-2-CAS-2017-14].Scopu