An ultra scale-down method is described to determine the response of cells to recovery by dead-end (batch) centrifugation under commercially defined manufacturing conditions. The key variables studied are the cell suspension hold time prior to centrifugation, the relative centrifugal force (RCF), time of centrifugation, cell pellet resuspension velocities, and number of resuspension passes. The cell critical quality attributes studied are the cell membrane integrity and the presence of selected surface markers. Greater hold times and higher RCF values for longer spin times all led to the increased loss of cell membrane integrity. However, this loss was found to occur during intense cell resuspension rather than the preceding centrifugation ...
A new method for altering red cell morphology by high-speed centrifugation of cells through a physio...
Large scale continuous cell���line cultures promise greater reproducibility and efficacy for the pro...
Enhanced sedimentation on inclined surfaces has been exploited successfully in mammalian cell cultur...
ABSTRACT: An ultra scale-down method is described to determine the response of cells to recovery by ...
Tools that allow cost-effective screening of the susceptibility of cell lines to operating condition...
Centrifugation is a unit operation used within a wide range of bioprocesses, including the productio...
The ability to recover cells from a fermentation broth in an intact form can be an important criteri...
With the potential to provide a cure as oppose to a treatment, human cell therapies offer an excitin...
In order to realise the potential of embryonic stem (ES) cells as a regenerative medicine, it is cru...
The scale-up and manufacturing of therapies based on intact whole cells presents a major challenge f...
Increases in mammalian cell culture titres and densities have placed significant demands on primary ...
The scale-up and manufacture of therapies based on intact whole cells presents a major challenge for...
Current challenge in a FDA approved cell therapy product for adult B-cell leukemia is reported to be...
This thesis describes the use of ultra scale-down (USD) methods to address the primary recovery chal...
Two dimensional (2-D) cell culture technologies have proven inadequate for large scale manufacturing...
A new method for altering red cell morphology by high-speed centrifugation of cells through a physio...
Large scale continuous cell���line cultures promise greater reproducibility and efficacy for the pro...
Enhanced sedimentation on inclined surfaces has been exploited successfully in mammalian cell cultur...
ABSTRACT: An ultra scale-down method is described to determine the response of cells to recovery by ...
Tools that allow cost-effective screening of the susceptibility of cell lines to operating condition...
Centrifugation is a unit operation used within a wide range of bioprocesses, including the productio...
The ability to recover cells from a fermentation broth in an intact form can be an important criteri...
With the potential to provide a cure as oppose to a treatment, human cell therapies offer an excitin...
In order to realise the potential of embryonic stem (ES) cells as a regenerative medicine, it is cru...
The scale-up and manufacturing of therapies based on intact whole cells presents a major challenge f...
Increases in mammalian cell culture titres and densities have placed significant demands on primary ...
The scale-up and manufacture of therapies based on intact whole cells presents a major challenge for...
Current challenge in a FDA approved cell therapy product for adult B-cell leukemia is reported to be...
This thesis describes the use of ultra scale-down (USD) methods to address the primary recovery chal...
Two dimensional (2-D) cell culture technologies have proven inadequate for large scale manufacturing...
A new method for altering red cell morphology by high-speed centrifugation of cells through a physio...
Large scale continuous cell���line cultures promise greater reproducibility and efficacy for the pro...
Enhanced sedimentation on inclined surfaces has been exploited successfully in mammalian cell cultur...