This thesis outlines the effects of ageing, calorie restriction and ageing-associated diseases on the mitochondrial proteome. Several techniques were tested to study the mitochondrial proteome: Techniques for visualisation of proteins after two dimensional (2D) blue native (BN) / sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (PAGE) were compared: staining of gels with Coomassie Brilliant Blue G-250 (CBBG), silver and the fluorescent dye SYPRO®Ruby as well as labelling of the proteins before gel run with fluorescent dyes from Refraction-2D™ Labeling Kit (differential gel electrophoresis, DIGE). A recommendation was elaborated either for staining with fluorescent dye, when enough amount of protein is available, or for labe...
Abstract: Mitochondrial dysfunction and impairment of the ubiquitin proteasome system have been desc...
AbstractAging is accompanied by the gradual deterioration of cell functions. Particularly, mitochond...
Increasing evidence supports the notion that increased oxidative stress is a fundamental cause in th...
This thesis outlines the effects of ageing, calorie restriction and ageing-associated diseases on th...
Mitochondrial dysfunction is evident in numerous neurodegenerative and age-related disorders. It has...
Cognitive decline coupled with functional loss of skeletal muscle strength is a quietly devastating ...
Understanding molecular processes underlying ageing still remains a challenge. Besides genetic deter...
AbstractMitochondria are dynamic organelles critical for many cellular processes, including energy g...
One of the greatest achievements of the past 150 years is the extraordinary extension of average hum...
Mitochondria are dynamic organelles critical for many cellular processes, including energy generatio...
Aging of mammalian brain is associated with a continuous decrease of the capacity to produce ATP by ...
The deleterious effects of aging on the brain remain to be fully elucidated. In the present study, p...
Zusammenfassend ist festzustellen, dass ein neues Proteomikverfahren für eine globale und quantitati...
Qualitative and quantitative changes of mitochondrial membrane proteins during aging were investigat...
Alterations in mitochondrial bioenergetics have been associated with brain aging. In order to evalua...
Abstract: Mitochondrial dysfunction and impairment of the ubiquitin proteasome system have been desc...
AbstractAging is accompanied by the gradual deterioration of cell functions. Particularly, mitochond...
Increasing evidence supports the notion that increased oxidative stress is a fundamental cause in th...
This thesis outlines the effects of ageing, calorie restriction and ageing-associated diseases on th...
Mitochondrial dysfunction is evident in numerous neurodegenerative and age-related disorders. It has...
Cognitive decline coupled with functional loss of skeletal muscle strength is a quietly devastating ...
Understanding molecular processes underlying ageing still remains a challenge. Besides genetic deter...
AbstractMitochondria are dynamic organelles critical for many cellular processes, including energy g...
One of the greatest achievements of the past 150 years is the extraordinary extension of average hum...
Mitochondria are dynamic organelles critical for many cellular processes, including energy generatio...
Aging of mammalian brain is associated with a continuous decrease of the capacity to produce ATP by ...
The deleterious effects of aging on the brain remain to be fully elucidated. In the present study, p...
Zusammenfassend ist festzustellen, dass ein neues Proteomikverfahren für eine globale und quantitati...
Qualitative and quantitative changes of mitochondrial membrane proteins during aging were investigat...
Alterations in mitochondrial bioenergetics have been associated with brain aging. In order to evalua...
Abstract: Mitochondrial dysfunction and impairment of the ubiquitin proteasome system have been desc...
AbstractAging is accompanied by the gradual deterioration of cell functions. Particularly, mitochond...
Increasing evidence supports the notion that increased oxidative stress is a fundamental cause in th...