The use of morphogenic suspension cultures for the development of a protoplast regeneration system in lily

The present study reports data on the development of a protoplast regeneration procedure in lily. Established morphogenic suspension cultures were obtained from callus cultures induced on mature embryos from crosses between cultivars of L. longiflorum. The effect on the frequency of protoplast division and microcolony formation of preculture conditions and origin of suspension cultures, composition of the protoplast isolation and culture media, anti-oxidants, an ethylene inhibitor, fetal calf serum, conditioned medium or a feeder culture and culture conditions are discussed