Add to ORKGUsing pIJ486 (Ward et al., 1986) two bifunctional (E. coli/Streptomvces) expression vectors were constructed. They contained the promoters lac (pGLW49) and tac (pGLW50) just upstream of a promoter-less kanamycin phosphotransferase gene (aphll)
Plasmid pSPNl is a 26.5kb cryptic plasmid, originally isolated from Streptomyces penemafaciens ATCC ...
By using the promoter-probe plasmid pIJ424, genomic DNA fragments of Actinomadura R39 were shown to ...
Experiments requiring strong repression and precise control of cloned genes can be difficult to cond...
A high-copy-number plasmid expression vector (pIJ6021) was constructed that contains a thiostrepton-...
At the onset of this thesis the aim was to design and develop an expression system capable of produc...
Various traits of Lactococcus lactis important for dairying are encoded by unstable plasmids. The us...
Shuttle vectors have been constructed which are suitable both for the selection of regulatory sequen...
A plasmid (pGEM-SD) was constructed in which the Shine-Dalgarno sequence had been deleted from pGEM-...
The 5.5 kb high-copy number cryptic plasmid pDI25 from Lactococcus lactis subsp. lactis 5136 was iso...
The cryptic Streptococcus cremoris Wg2 plasmid pWV01 (1.5 megadaltons) was genetically marked with t...
We have constructed two plasmid vectors for cloning and expression of DNA fragments controlled by th...
An integration vector, pORI13, was developed to screen in Lactococcus lactis for expression signals ...
Plasmids were prepared by inserting genomic DNA fragments from Streptomyces cacaoi within the mel ge...
Bibliography: pages 147-177.Nocardioforms are Gram-positive, aerobic actinomycetes and are a metabol...
We developed a generic method for high-throughput cloning in bacteria that are less amenable to conv...
Plasmid pSPNl is a 26.5kb cryptic plasmid, originally isolated from Streptomyces penemafaciens ATCC ...
By using the promoter-probe plasmid pIJ424, genomic DNA fragments of Actinomadura R39 were shown to ...
Experiments requiring strong repression and precise control of cloned genes can be difficult to cond...
A high-copy-number plasmid expression vector (pIJ6021) was constructed that contains a thiostrepton-...
At the onset of this thesis the aim was to design and develop an expression system capable of produc...
Various traits of Lactococcus lactis important for dairying are encoded by unstable plasmids. The us...
Shuttle vectors have been constructed which are suitable both for the selection of regulatory sequen...
A plasmid (pGEM-SD) was constructed in which the Shine-Dalgarno sequence had been deleted from pGEM-...
The 5.5 kb high-copy number cryptic plasmid pDI25 from Lactococcus lactis subsp. lactis 5136 was iso...
The cryptic Streptococcus cremoris Wg2 plasmid pWV01 (1.5 megadaltons) was genetically marked with t...
We have constructed two plasmid vectors for cloning and expression of DNA fragments controlled by th...
An integration vector, pORI13, was developed to screen in Lactococcus lactis for expression signals ...
Plasmids were prepared by inserting genomic DNA fragments from Streptomyces cacaoi within the mel ge...
Bibliography: pages 147-177.Nocardioforms are Gram-positive, aerobic actinomycetes and are a metabol...
We developed a generic method for high-throughput cloning in bacteria that are less amenable to conv...
Plasmid pSPNl is a 26.5kb cryptic plasmid, originally isolated from Streptomyces penemafaciens ATCC ...
By using the promoter-probe plasmid pIJ424, genomic DNA fragments of Actinomadura R39 were shown to ...
Experiments requiring strong repression and precise control of cloned genes can be difficult to cond...