Studies of the Glyoxylate Bypass of Streptomyces coelicolor A3(2)

In this study, molecular cloning of the structural gene encoding malate synthase (aceB) from Streptomyces coelicolor was attempted using two different approaches. Despite identification of potential sequences using genetic fingerprinting and southern hybridisation techniques, all my attempts to clone the full coding sequence of the gene were unsuccessful. Analysis of DNA sequences upstream of the structural gene encoding isocitrate lyase (aceA), led to the identification of potential open reading frame (pORF) which shows a very close similarity to pORF from Mycobacterium tuberculosis. The function of such an ORF in both organisms remains to be discovered