In vitro propagation of Camellia fascicularis: a plant species with extremely small populations

Camellia fascicularis is an endangered evergreen ornamental plant with pale yellow flowers. An efficient and reproducible in vitro regeneration method is required for its large-scale propagation and germplasm conservation. In this study, one axillary buds per nodal stem were obtained from C. fascicularis cultured on Murashige and Skoog (MS) medium containing 0.1 mg/L of indole-3-acetic acid (IAA) combined with 1.0 of mg/L 6-benzylaminopurine (BA). Axillary buds from the stem segments were transferred to the modified woody plant medium (WPM) supplemented with 3.0 mg/L of BA in combination with 0.3 mg/L of IAA for multiplication, thereby resulting in the highest shoot multiplication rate of 6.8. Multiple shoots were divided into nodal stems and shoot tips and were induced to root. The shoot tips were induced to root by culturing on 1/2 MS medium supplemented with 2.0 mg/L of indole-3-butyric acid (IBA) in combination with 0.3 mg/L of α-naphthalene acetic acid (NAA), which resulted in 76.0% rooting efficiency with 2.3 roots per shoot. The optimal hormone ratio for inducing root of nodal stems was 1.0 mg/L of IBA in combination with 2.0 mg/L of NAA, which produced 72.7% rooting efficiency with 1.7 roots per nodal stem. These two rooted plantlets were successfully acclimatized and established in a greenhouse.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author