Transgenic Miscanthus lutarioriparius that co-expresses Cry 2Aa# and Bar genes

Miscanthus lutarioriparius, a domestic species of China, belongs to the genus of perennial rhizomatous Miscanthus and is a promising energy crop for biomass production. In order to establish an Agrobacterium tumefaciens transformation protocol and obtain transgenic plants co-expressing the Cry 2Aa# and Bar genes, an Agrobacterium-mediated transformation method for M. lutarioriparius was developed in this study. To inhibit tissue browning, optimized callus induction and regeneration media were developed. Embryogenic calli were efficiently induced from immature inflorescences on Murashige and Skoog (MS) medium containing 0.2 g L-1 citric acid (CA). Agrobacterium tumefaciens EHA105 carrying pC3300 with Cry 2Aa# and Bar genes were introduced into embryogenic calli. Six transgenic lines were selected by Basta screening and confirmed by genomic PCR. The herbicide tolerance of M. lutarioriparius transgenic plants was verified by spraying with glufosinate and expression of the Cry 2Aa# protein by ELISA. Therefore, an Agrobacterium-mediated transformation protocol for M. lutarioriparius was established and co â expression of herbicide tolerance and Cry 2Aa# was successfully introduced with the system.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author