Application of the Indicator Amino Acid Oxidation Technique to Determine the Metabolic Availability of Lysine in Millet

Application of the Indicator Amino Acid Oxidation Technique to Determine the Metabolic Availability of Lysine in Millet Abrar Fakiha Masters of Science Graduate Department of Nutritional Science University of Toronto 2019 Abstract Objective 1) Determine the metabolic availability (MA) of the most limiting indispensable amino acid, lysine, in millet.2) Assess the effect of complementing millet protein with lentils Method The MA of lysine in millet was studied using the indicator amino acid oxidation (IAAO) and slope ratio methods. Five healthy males participated in 8 experiments: 4 lysine intakes from a reference protein, 3 intakes from millet and 1 complementation experiment with lentils. The MA of lysine was estimated by comparing the reference protein slope with the millet slope using the slope ratio method Results The MA of lysine from millet was 97%. Complementation with lentils decreased the IAAO response suggesting increased uptake for protein synthesis Conclusion Lysine is completely bioavailable, however still limiting in millet. Complementation with lentils in 2:1 ratio is recommended to meet the lysine and protein requirement for adults consuming a millet based diet.M.Sc