Proteomics Analysis of Latex from Hevea brasiliensis (Clone RRIM 600)

The natural rubber latex extracted from the bark of Hevea brasiliensis plays various important roles in today's modern society. Following ultracentrifugation, the latex can be separated into 3 layers; C-serum, lutoids, and rubber particle. Previous studies showed that a large number of proteins were present inside these three layers. However, a complete proteome for this important plant is still unavailable. Protein sequences have been recently translated from the completed draft genome database of H. brasiliensis leading to the creation of annotated protein databases of H. brasiliensis biosynthetic pathways; photosynthesis, latex allergens, rubberwood formation, latex biosynthesis, and disease resistance. This research was conducted to identify the proteins contained within the latex by way of de novo sequencing from mass spectric data obtained from the 3 layers of the latex. Peptides from these proteins were fragmented using collision-induced dissociation (CID), higher-energy collisional dissociation (HCD) and electron-transfer dissociation (ETD) activation methods. A large percentage of proteins from the biosynthetic pathways were successfully identified, ranging from 63% to 100%. In addition, a total of 1839 unique proteins were identified from the whole translated draft genome database (AnnHBM).The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author