Primary Human Monocytes Differentiate into M2 Macrophages and Involve Notch-1 Pathway

The current study investigates whether inhibiting the Notch-1 signaling pathway in primary human monocytes enhances M2 macrophage differentiation. We generated primary human monocytes cell culture model to understand the effect of Notch-1 signaling pathway. Monocytes were treated with Notch-1inhibitors DAPT or siRNA. Our data show that there was a significant increase in the M1 macrophage population demonstrated by iNOS marker in the primary human monocytes treated with apoptotic conditioned medium (ACM). Next, the levels of pro-inflammatory cytokines IL-6, and MCP-1, as well as TNF-Îą increased in ACM media (pThe accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author