Fatty acids composition and mechanism of protective effects of myrtle berries seeds aqueous extract against alcohol-induced peptic ulcer in rat

This study aimed to investigate the anti-ulcer and antioxidant activities of the myrtle berries seeds aqueous extract (MBSAE) on peptic ulcer model induced by ethanol (EtOH) in male Wistar rats. MBSAE is rich in total polyphenols, total flavonoids and unsaturated fatty acids particularly linoleic (C18:2) and oleic acids (C18:1). MBSAE also exhibited an importance in vitro antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 172.1 Âľg/mL) and superoxide anion (IC50 = 200.24 Âľg/mL) scavenging activities. In vivo, MBSAE provide a dose-dependent protection against ethanol-induced gastric and duodenal macroscopic and histological alterations. Also, it inhibits the secretory profile disturbances and lipid peroxidation, preserve normal antioxidant enzyme activities and non-enzymatic antioxidant levels. More importantly, we showed that alcohol acute intoxication increased gastric and duodenal calcium, hydrogen peroxide (H2O2) and free iron levels while MBSAE treatment protected against intracellular mediators deregulation. In conclusion, we suggest that MBSAE had a potent protective effects against alcohol-induced peptic ulcer in rat. This protection might be related in to part its antioxidant properties as well as its opposite effects on some studied intracellular mediators.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author