Using Quantitative Synthetic Genetic Interaction Profiling in Escherichia coli to Investigate Bacterial Protein Synthesis

Bacterial protein synthesis depends on the coordination of a complex, multi-component machinery that processes ribosomal RNA, assembles ribosomes, and translates diverse messenger RNA transcripts to meet changing physiological and environmental demands. Despite decades of detailed investigations, which enabled basic mechanistic understanding of the core translation processes, the global functional connectivity and component memberships of this central system and its relationship to other biological pathways had remained unclear. Physical interaction data can suggest the biological roles and associations of gene products, but only genetic interaction (GI) screens can truly reveal pathway-level functional dependencies. For my Thesis, I performed quantitativePh.D.2016-11-30 00:00:0