Unravelling the effects of static magnetic field stress on cytosolic proteins ofS almonelle by a proteomic approach

Abstract The present study investigated the adaptation of Salmonella enterica subsp. enterica serovar Hadar to static magnetic field exposure (200 mT, 9 hours). The proteomic analysis provides an overview of potentially important cytosolic proteins that Salmonella needs to regulate in order to survive and adapt to magnetic stress. Via two dimensional electrophoresis (2-DE) and LC-MS/MS spectrometry, we compared cytosolic proteomes before and after exposure to magnetic field. A total of 35 proteins, displaying more than 2-fold change were differentially expressed in exposed cells among which 25 were upregulated and 10 downregulated. These proteins can be classified mainly into six categories: (i) proteins involved in metabolic pathways of carbohydrates, (ii) chaperones and proteins produced in response to oxidative stress, (iii) proteins involved in energy homeostasis, (iv) the elongation factors (EF-Tu and EF-Ts), (v) protein involved in motility and (vi) proteins involved in molecules transport. Many of the presented observations could be explained, while some represent still-unknown mechanisms. In addition, this study reveals four hypothetical proteins. It seems that the stress response to SMF (200 mT) is essentially set up to avoid oxidative damages with the over-expression of proteins directly involved in oxidative stress response and metabolic switches to counteract oxidative stress. Interestingly, several proteins induced under SMF exposure are found to overlap with those induced by other stresses such as heat shock and starvation.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author