An efficient temporary Immersion system for micropropagation of hybrid hazelnut

An efficient protocol for micropropagation of hybrid hazelnut (Corylus) was developed using Temporary Immersion System (TIS) for multiplication of shoots in the liquid medium. Growth and multiplication of shoots as well as the length and number of nodes in developing shoots were significantly improved by combining two forms of chelated iron, ethylenediamine-N,N'-di-(ortho-hydroxyphenyl) acetic acid (FeEDDHA) and FeSO4/EDTA (FeEDTA). The use of TIS with optimized concentrations of FeEDTA and FeEDDHA significantly increased the number of shoots, shoot height, leaf area and chlorophyll content in all of the four cultivars tested compared to semi-solid medium. Proliferated shoots developed roots on a semi-solid medium containing 2.5 µM IBA and rooted plantlets acclimatized in greenhouse conditions with 80% survival.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author