Study on the homology of the genomes of tetraploid Asiatic lilies (Lilium) using FISH (Fluorescence in situ hybridization)

Asiatic lily cultivars, bred by hybridization and/or chromosome doubling of species of section Sinomartagon of Lilium, are diploid, triploid or tetraploid, but the homology of the genomes among Sinomartagon species and Asiatic lilies remains unclear. In the present research, two tetraploid Asiatic cultivars were analyzed, using 45S rDNA as probe, for their FISH karyotypes and their chromosomal association, anaphase I, telophase II and pollen viability were surveyed to assess the multivalent segregation. Chromosomal assortment of six progenies of the two tetraploid cultivars were also investigated. The results showed that the tetraploid cultivars had similar FISH karyotypes, they predominantly formed multivalents, and these were equally separated because their anaphase I, telophase II and their pollen viability are normal, as those of diploid species; apart from minor variations, FISH karyotypes of progenies were similar to each other and to their parents. Based on these results and considering the high crossability among Sinomartagon species and/ or Asiatic lilies, we concluded that Sinomartagon species and their resulting cultivars share a common genome, so polyploid Asiatic lilies are autopolyploid.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author