The organotypic hippocampal slice culture technique is an in vitro system for explanting the hippocampus and incubating tissue for up to four weeks. This method is useful because the tissue is maintained in a state closely resembling what is found in vivo. Previous studies have used organotypic cultures to study hippocampal physiology, but attempts to use the model specifically for adult neurogenesis research have not convincingly shown that newborn neurons can be efficiently studied and quantified.In this thesis, we optimized organotypic slice cultures for neurogenesis research. We used the model to study extrasynaptic GABAergic signaling in newborn dentate granule neurons. Following treatment with a selective extrasynaptic GABAAR agonist,...
BACKGROUND: Stem cell cultures are key tools of basic and applied research in Regenerative Medicine....
One of the goals in neuroscience is to obtain tractable laboratory cultures that closely recapitulat...
Although the majority of human illnesses occur during adulthood, most of the available in vitro dise...
The organotypic hippocampal slice culture technique is an in vitro system for explanting the hippoca...
The function of the hippocampus depends on the process of adult hippocampal neurogenesis which under...
The neurosphere assay is the most widely used in vitro tool to determine the proliferative and diffe...
The neurosphere assay is the most widely used in vitro tool to determine the proliferative and diffe...
The neurosphere assay is the most widely used in vitro tool to determine the proliferative and diffe...
Adult neurogenesis in the dentate gyrus is assuming an increasingly important role in supporting hip...
Adult neurogenesis in the dentate gyrus is assuming an increasingly important role in supporting hip...
The use of organotypic hippocampal slice culture (OHSC) has become a powerful tool for studying cell...
To establish a method of organotypic cerebral culture. So as to pave the way for building some neuro...
One of the most significant barriers towards translational neuropsychiatry would be an unavailabilit...
systems while still providing ease of use in the lab. Because neurons can exist in the body over a ...
systems while still providing ease of use in the lab. Because neurons can exist in the body over a ...
BACKGROUND: Stem cell cultures are key tools of basic and applied research in Regenerative Medicine....
One of the goals in neuroscience is to obtain tractable laboratory cultures that closely recapitulat...
Although the majority of human illnesses occur during adulthood, most of the available in vitro dise...
The organotypic hippocampal slice culture technique is an in vitro system for explanting the hippoca...
The function of the hippocampus depends on the process of adult hippocampal neurogenesis which under...
The neurosphere assay is the most widely used in vitro tool to determine the proliferative and diffe...
The neurosphere assay is the most widely used in vitro tool to determine the proliferative and diffe...
The neurosphere assay is the most widely used in vitro tool to determine the proliferative and diffe...
Adult neurogenesis in the dentate gyrus is assuming an increasingly important role in supporting hip...
Adult neurogenesis in the dentate gyrus is assuming an increasingly important role in supporting hip...
The use of organotypic hippocampal slice culture (OHSC) has become a powerful tool for studying cell...
To establish a method of organotypic cerebral culture. So as to pave the way for building some neuro...
One of the most significant barriers towards translational neuropsychiatry would be an unavailabilit...
systems while still providing ease of use in the lab. Because neurons can exist in the body over a ...
systems while still providing ease of use in the lab. Because neurons can exist in the body over a ...
BACKGROUND: Stem cell cultures are key tools of basic and applied research in Regenerative Medicine....
One of the goals in neuroscience is to obtain tractable laboratory cultures that closely recapitulat...
Although the majority of human illnesses occur during adulthood, most of the available in vitro dise...