Biological parameters and molecular markers of clone CL Brener - the reference organism of the Trypanosoma cruzi Genome Project

Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 92(6), November/December 1997, pp. 811-814 Biological parameters and molecular markers of clone CL Brener - the reference organism of the Trypanosoma cruzi Genome Project Bianca Zingales, Maria Elizabeth S Pereira, Katia A Almeida, Eufrosina S Umezawa, Nedia S Nehme, Riva P Oliveira, Andrea Macedo, Ricardo P Souto Code Number:OC97152 Sizes of Files: Text: 16.9K Graphics: Tables (jpg) - 31.1K Line drawings and photographs (jpg) - 43.1K Clone CL Brener is the reference organism used in the Trypanosoma cruzi Genome Project. Some biological parameters of CL Brener were determined: (a) the doubling time of epimastigote forms cultured in liver infusion-tryptose (LIT) medium at 28 C is 58+/-13 hr; (b) differentiation of epimastigotes to metacyclic trypomastigotes is obtained by incubation in LIT-20% Grace's medium; (c) trypomastigotes infect mammalian cultured cells and perform the complete intracellular cycle at 33 and 37 C; (d) blood forms are highly infective to mice; (e) blood forms are susceptible to nifurtimox and benznidazole. The molecular typing of CL Brener has been determined: (a) isoenzymatic profiles are characteristic of zymodeme ZB; (b) PCR amplification of a 24Sa ribosomal RNA sequence indicates it belongs to T. cruzi lineage 1; (c) schizodeme, randomly amplified polymorphic DNA (RAPD) and DNA fingerprinting analyses were performed. Key words: CL Brener - Trypanosoma cruzi - Genome Project - biological characteristics - molecular typing Copyright 1997 Fundacao Oswaldo Cru