Cloning of sinefungin biosynthetic genes from Streptomyces incarnatus to Streptomyces lividans

Actinomycetes, 1992 Vol.3 Part 2 25-30 CLONING OF SINEFUNGIN BIOSYNTHETIC GENES FROM STREPTOMYCES INCARNATUS TO STREPTOMYCES LIVIDANS HALINA MALINA(1), FRANCOISE LAWRENCE, and MALKA ROBERT-GERO Code Number: AC95006 Sizes of Files: Text: 20K Graphics: Line Drawings (gif) 31K Photos (jpg) 21K / Halftones (gif) 33K ABSTRACT. Biosynthetic genes (sin genes) of sinefungin, a transmethylase inhibitor, antifungal and antiparasitic nucleoside antibiotic produced by Streptomyces incarnatus, were shotgun cloned into Streptomyces lividans. The genes are clustered on a 14 kb fragment of DNA. The expression of the sin gene cluster in S. lividans leads to the production of a sinefungin-like antibiotic. The compound has antifungal and antiparasitic activities in vitro. The expression of the gene cluster in S.lividans and E. coli causes an astringent-like response. Copyright 1992 CET