Due to the overlapping distribution of Trypanosoma rangeli and T. cruzi in Central and South America, sharing several reservoirs and triatomine vectors, we herein describe a simple method to collect triatomine feces and hemolymph in filter paper for further detection and specific characterization of these two trypanosomes. Experimentally infected triatomines feces and hemolymph were collected in filter paper and specific detection of T. rangeli or T. cruzi DNA by polymerase chain reaction was achieved. This simple DNA collection method allows sample collection in the field and further specific trypanosome detection and characterization in the laboratory
Three synthetic oligonucleotides corresponding to sequences within the D7a divergent domain of the l...
A rapid DNA extraction was used for T. cruzi detection in triatomines dry fecal spots collected on f...
Triatomine vectors were collected on human dwellings in Michoac�n M�xico. Blood meal sources were id...
Due to the overlapping distribution of Trypanosoma rangeli and T. cruzi in Central and South America...
Due to the overlapping distribution of Trypanosoma rangeli and T. cruzi in Central and South America...
Due to the overlapping distribution of Trypanosoma rangeli and T. cruzi in Central and South America...
A rapid DNA extraction was used for T. cruzi detection in triatomines dry fecal spots collected on f...
A rapid DNA extraction was used for T. cruzi detection in triatomines dry fecal spots collected on f...
A rapid DNA extraction was used for T. cruzi detection in triatomines dry fecal spots collected on f...
A rapid DNA extraction was used for T. cruzi detection in triatomines dry fecal spots collected on f...
We designed a PCR assay targeted on repeated elements of the ribosomal intergenic spacer which produ...
We used the species specificity and repetitious nature of subtelomeric kinetoplastida sequences to g...
We designed a PCR assay targeted on repeated elements of the ribosomal intergenic spacer which produ...
Abstract. For effective control programs, accurate assessment of Trypanosoma cruzi infection in vect...
The polymerase chain reaction was used to amplify the highly variable region of the kinetoplast mini...
Three synthetic oligonucleotides corresponding to sequences within the D7a divergent domain of the l...
A rapid DNA extraction was used for T. cruzi detection in triatomines dry fecal spots collected on f...
Triatomine vectors were collected on human dwellings in Michoac�n M�xico. Blood meal sources were id...
Due to the overlapping distribution of Trypanosoma rangeli and T. cruzi in Central and South America...
Due to the overlapping distribution of Trypanosoma rangeli and T. cruzi in Central and South America...
Due to the overlapping distribution of Trypanosoma rangeli and T. cruzi in Central and South America...
A rapid DNA extraction was used for T. cruzi detection in triatomines dry fecal spots collected on f...
A rapid DNA extraction was used for T. cruzi detection in triatomines dry fecal spots collected on f...
A rapid DNA extraction was used for T. cruzi detection in triatomines dry fecal spots collected on f...
A rapid DNA extraction was used for T. cruzi detection in triatomines dry fecal spots collected on f...
We designed a PCR assay targeted on repeated elements of the ribosomal intergenic spacer which produ...
We used the species specificity and repetitious nature of subtelomeric kinetoplastida sequences to g...
We designed a PCR assay targeted on repeated elements of the ribosomal intergenic spacer which produ...
Abstract. For effective control programs, accurate assessment of Trypanosoma cruzi infection in vect...
The polymerase chain reaction was used to amplify the highly variable region of the kinetoplast mini...
Three synthetic oligonucleotides corresponding to sequences within the D7a divergent domain of the l...
A rapid DNA extraction was used for T. cruzi detection in triatomines dry fecal spots collected on f...
Triatomine vectors were collected on human dwellings in Michoac�n M�xico. Blood meal sources were id...