Role of the aromatic hydrocarbon receptor in the suppression of cytochrome P450 2C11 by polycyclic aromatic hydrocarbons

grantor: University of TorontoThe aromatic hydrocarbon receptor (AHR) mediates the induction of cytochromes P450 (CYP) of the CYP1A subfamily caused by polycyclic aromatic hydrocarbons (PAHs). CYP1A induction by PAHs is accompanied by down-regulation of CYP2C11, the predominant CYP expressed constitutively in the liver of male rats. We performed a structure-activity relationship study with a series of PAHs of the anthracene class in order to determine if the AHR is involved in CYP2C11 down-regulation. Anthracene, benz (a) anthracene, dibenz (a,c) anthracene, dibenz (a,h) anthracene, 7,12-dimethylbenz (a) anthracene, as well as 2,3,7,8-tetrachlorodibenzo-p-dioxin and 3-methylcholanthrene decreased CYP2C11 immunoreactive protein levels to varying degrees in primary rat hepatocytes cultured on a laminin-rich extracellular matrix. The binding affinity of the PAHs for the rat liver cytosolic AHR correlated with the potency for transforming the cytosolic AHR to its DNA-binding form. In addition, the ability of the PAHs to suppress CYP2C11 correlated with both the AHR binding affinity and the AHR transformation potency. These results suggest that the AHR plays a role in the down-regulation of CYP2C11 caused by PAHs.M.Sc