Interplay of J chain and disulfide bonding in assembly of polymeric IgM

grantor: University of TorontoNormal mouse IgM is synthesized as hexamers in the absence of J chain and as pentamers in its presence. Previous work has suggested that polymer size is also closely related to formation of the inter-$\mu$ chain disulfide bond mediated by cysteine 414, one of three cysteines involved in inter-$\mu$ chain bonding. This correlation in turn suggested that formation of C414-C414 might be required for J chain to influence how IgM assembles and that formation of C414-C414 might affect the J chain/IgM stoichiometry. To test such hypotheses we have used cell-lines that either express or do not express J chain to produce IgM in which serine was substituted for C414. In contrast to the case of IgM assembled from normal $\mu$ chains, IgM-S414 was secreted mostly as pentamers and tetramers but not as hexamers, irrespective of J chain synthesis. These results indicate that the role of J chain as modulator of IgM structure and function requires C414. Moreover, a more detailed analysis of the structure of J-plus and J-minus IgM-S414 revealed that J chain, in fact, influenced the nature of secreted IgM-S414: in the absence of J chain, some IgM-S414 was secreted as dimers and trimers, while in the presence of J chain, some IgM was secreted as non-covalently assembled pentamers. These results imply that disulfide bonding can occur differently from the pattern depicted in conventional models of IgM structure.M.Sc