Pharmacological effects of low- dose of aspirin on Corpus Luteum functions in mature cycling female mice

Objective: To investigate long-term effect of aspirin in low-dose on the corpus luteum functions and its hormonal changes associated with ovarian and uterine structural changes. Design: Prospective study. Setting: Institute of the Embryo Research and Infertility Treatment- University of Baghdad. Materials and methods: In the treatment group, 24 mature cycling female mice underwent subcutaneous administration of aspirin at a dose level of (7.5mg/Kg b.w)twice daily at the beginning of diestrous phase of the estrous cycle. In the control group, 24 mature cycling female mice underwent subcutaneous administration of a placebo(distilled water) twice daily at the beginning of diestrous phase of the estrous cycle. Main outcome measure: Uterine and ovarian morphological changes, uterine and ovarian weight changes, serum level of (FSH,LH&Progesterone) and ovarian and uterine structural changes. Results: There was statistically significant increase in progesterone level, number of corpora lutea, diameter of granulosa cells. and a significant decrease in gonadotropins (FSH/LH) , number of growing follicles, uterine weight, endometrial living cell height, endometrial and myometrial thickness, diameter of endometrial glands. Conclusion(s): long-term administration of a low-dose of aspirin to mice at the beginning of diestrous phase, causes the following changes: significant decrease in uterine weight with development of hemorrhagic spots on the external surface of uterine horns of only those animals that receive treatment for 30 days, and a significant decrease in serum level of both gonadotropins (FSH/LH) associated with significant increase in progesterone level, number of corpora lutea, diameter of granulosa cells, congestion in the uterus, ovary and prolongation of the luteal phase in all 4 periods of treatment