Oxygen uptake kinetics of Pseudomonas sp. strain VB grown under limiting concentration of 2-methoxyethanol

Oxygen uptake and 2-methoxyethanol oxidation kinetics of 2-methoxyethanol degrading bacterium Pseudomonas sp. strain VB were studied. The bacterium was grown under oxygen saturating conditions with limiting concentrations of 2-methoxyethanol. Pseudomonas sp. strain VB was found to utilize one mole of oxygen per one mole of 2-methoxyethanol as opposed to four moles of oxygen required for total oxidative metabolism. This indicates that intermediate metabolites are involved during the degradation of 2-methoxyethanol under aerobic conditions