Bioengineering cellulose-hemicellulose networks in plants

The interactions between cellulose and hemicellulose in the cell walls are important in the industrial application of the cellulose (natural) fibres. We strive to modify these interactions (i) by interfering with cellulose biosynthesis and (ii) by direct interference of the interactions through the use of carbohydrate binding modules (CBMs). Additionally, we strive to establish a link between the putative expansin CBM and cell wall/plant modification. The investigation generates structural changes in the cell walls leading to altered cellular, organ and whole plant morphology. The results demonstrate that a gain of control over the inherent cellulose fibre characteristics for paper and textile applications is possible in the future. This can complement the current use of enzymes and chemicals for surface modification of cellulose fibre. The approaches described in the thesis hold great promise for modifying the fibre properties inside the plant. The technology can be transferred to economically important fibre species, e.g. flax, hemp and poplar