Purification of a Thermostable DNA Polymerase from a Thermotoga Species
- Simpson, H.D.
- Coolbear, Tim
- Daniel, Roy M.
Publication date
January 1990
DOI Publisher
Wiley ISSN
0077-8923 Citation count (estimate)
3Abstract
Most of the initial studies on the fundamental cellular process of biosynthesis and repair of deoxyribonucleic acid (DNA) have been carried out on the bacterium Escherichia coli.¹When a single-stranded DNA template is provided with a primer, the 5’ - 3’ polymerase copies the template in the presence of deoxyribonucleotide triphosphates and a divalent cation, for example, Mg²⁺
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