Uji Penghambatan Aktivitas alfa-glukosidase Ekstrak dan Fraksi Daun Antidesma montanum Blume

alpha-Glucosidase inhibitor has known to be a therapeutic agent for diabetes mellitus (DM)treatment, especially type 2 DM. Based on previous studies. There are various plantsthat have the effect of inhibiting the activity of a-glucosidase, one of which is garuleaves (Antidesma montanum Blume). This research aimed to get the fraction whichhad the highest Il-glucosidase inhibiting activity from ethanol extract of garu leavesand identify the chemical compounds from the most active fraction. Simplisia powderwas extracted by maseration using 80% ethanol then fractionated using n-hexane, ethylacetate, and methanol. Inhibitory activity test was performed by measuring absorbanceof p-nitrophenol, which produced by reaction between Il-glucosidase and p-nitrophenyl-u-l)-glucopyranoside, using microplate reader at 405 nm. The result showed that ethylacetate fraction have the best Il-glucosidase inhibitory activity with IC50 values 138.38ppm. The test of enzyme kinetics showed that ethyl acetate fraction inhibited competitively.The phytochemical screening showed that ethyl acetate fraction of garu leaves containedglycosides, tannins, and terpenes