Mechanistic interrogations of ribosomal and non-ribosomal natural product enzymes

Described herein is my thesis work towards structural and biochemical characterization of bacterial proteins involved in metabolite regulation and natural product biosynthesis. In the first case, I will describe the structural basis for transcriptional regulation of a small molecule metabolite, p-coumarate, and the remaining cases involve enzymes responsible for biosyntheses of complex natural products. Among the natural product cases are those falling under the class of ribosomally synthesized and post-translationally modified peptides (RiPPs), with a particular emphasis on the thiopeptide subclass of RiPPs. Additionally, characterization of an off-loading enzyme involved in hybrid polyketide/non-ribosomal peptide (PK-NRP) natural product biosynthesis is described.LimitedAuthor requested closed access (OA after 2yrs) in Vireo ETD syste