Yeast Mutants That Require the GPI Biosynthetic Gene GPI1 for Growth

155 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2000.The isolation of t-s smp3, gaa1, and gpi2 strains using a synthetic lethality screen demonstrates that this is a valid approach to identify genes involved in GPI anchor biosynthesis. This suggests that many of the remaining S.L. mutants are also likely to harbor mutations in GPI and cell wall assembly. Strategies to identify the remaining non- t-s/S.L. mutations include complementation of other conditional growth phenotypes (e.g. CFW hypersensitivity) or the non-sectoring phenotype.U of I OnlyRestricted to the U of I community idenfinitely during batch ingest of legacy ETD