DNA and Ligand Binding and Kinetic Properties of Wild-Type and Mutant RsrI [n6-Adenine] DNA Methyltransferases

183 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2000.To complement the biochemical characterization of M•Rsr I in vitro, DNA binding analysis in vivo was performed using the bacteriophage P22-based challenge-phage assay. I isolated a catalytically impaired mutant, M•RsrI (L72P), that, unlike wild-type M•RsrI, binds in a methylation-sensitive, site-specific manner to DNA.U of I OnlyRestricted to the U of I community idenfinitely during batch ingest of legacy ETD