Studies on the Proton Pumping Mechanism of Aa(3)-Type Cytochrome C Oxidase

192 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2006.The monomeric form of bovine heart mitochondrial cytochrome c oxidase was used to detect directly the proton uptake and release events during the F-to-O transition. Absorption change of the pH sensitive dye was monitored spectroscopically. Rapid proton release followed by slow proton uptake was observed. This result indicates that both the pumped protons and the protons required for chemistry at the active site might come from an internal proton pool containing at least two protons.U of I OnlyRestricted to the U of I community idenfinitely during batch ingest of legacy ETD