Design, Syntheses and Functional Studies of DNA Binding Metal Complexes

158 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998.Both cis- and trans- (Pt(NH$\sb3)\sb2$(4-Me-Py)Cl$\rbrack\sp+$ bind DNA covalently at the N7-site of the guanines forming a monodentate adduct, but only the cis-isomer has anticancer activity. The interactions of these platinum complexes with the DNA-heptamer d(CCTGTCC):d(GGACAGG) were examined and a NMR based high resolution structure was determined. Surprisingly both platinum-DNA complexes do not form a very stable complex with its complementary strand. Over time the platinum-DNA complex decomposes and either the 2-methyl-pyridinium group or the platinum complex itself is detached. No interstrand crosslinking was observed.U of I OnlyRestricted to the U of I community idenfinitely during batch ingest of legacy ETD