Three-Dimensional Microelectrode Array for Recording Dissociated Neuronal Cultures

97 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2008.This work demonstrates the design, fabrication, packaging, characterization, and functionality of an electrically and fluidically active three-dimensional microelectrode array (3D MEA). The 3D MEA consists of a stack of individually patterned thin films that form a cell chamber conducive to maintaining and recording the electrical activity of a long-term three-dimensional network of rat cortical neurons. Silicon electrode layers contain a polymer grid that is conducive to branching, growth, and network formation of the neurons. Along the walls of these electrode layers lie exposed gold electrodes which permit recording and stimulation of the neuronal electrical activity. Silicone elastomer microfluidic layers provide an artificial vasculature for nutrient supply and aeration, as well as permit the loading of dissociated neurons into the structure. The fluidic layers also serve as insulation for the microelectrodes. Cells have been shown to survive in the 3D MEA for up to 28 days, with spontaneous and evoked electrical recordings performed in that time. This device has also been used to perform a drug test, yielding an additional capability for this device.U of I OnlyRestricted to the U of I community idenfinitely during batch ingest of legacy ETD