Complete genome sequence and construction of an infectiousfull?length cDNA clone of a German isolate of celery mosaic virus

AbstractThe complete genome sequence of a German isolate of celery mosaic virus (CeMV, a potyvirus) from Quedlinburg (DSMZPV-1003) was determined (MF962880). This represents the second fully sequenced genome of this virus, along with a Californianisolate (HQ676607.1). The positive-sense single-stranded RNA is 10,000 nucleotides in length and shows the typicalorganization of potyviruses but has a shorter PIPO than CeMV California. In comparison to CeMV isolates from differentorigins, CeMV-Quedlinburg and isolates from the Netherlands (AF203531.1) and Aschersleben, Germany (AJ271087.1)show a NAG instead of DAG in the region of the coat protein responsible for aphid transmission. In this study the firstinfectious full-length clone of celery mosaic virus was obtained and the infectivity confirmed by Rhizobium radiobacterinfiltration of Apium species