Molecular characterization of a novel luteovirus infecting appleby next?generation sequencing

Abstract A new single-stranded positive-sense RNAvirus, which shares the highest nucleotide (nt) sequenceidentity of 53.4% with the genome sequence of cherry-associatedluteovirus South Korean isolate (ChALV-SK, genusLuteovirus), was discovered in this work. It is provisionallynamed apple-associated luteovirus (AaLV). The completegenome sequence of AaLV comprises 5,890 nt and containseight open reading frames (ORFs), in a very similar arrangementthat is typical of members of the genus Luteovirus.When compared with other members of the family Luteoviridae,ORF1 of AaLV was found to encompass anotherORF, ORF1a, which encodes a putative 32.9-kDa protein.The ORF1-ORF2 region (RNA-dependent RNA polymerase,RdRP) showed the greatest amino acid (aa) sequence identity(59.7%) to that of cherry-associated luteovirus Czech Republic isolate (ChALV-CZ, genus Luteovirus). The resultsof genome sequence comparisons and phylogenetic analysis,suggest that AaLV should be a member of a novel speciesin the genus Luteovirus. To our knowledge, it is the sixthmember of the genus Luteovirus reported to naturally infectrosaceous plant