Sequence analysis of six full?length bean yellow mosaic virusgenomes reveals phylogenetic diversity in India strains, suggestingsubdivision of phylogenetic group?IV

Abstract We report the complete genome sequence offive bean yellow mosaic virus (BYMV) isolates (CK-GL1,CK-GL3, CK-GL4, CK-GL5 and Vfaba2) that share 74.6-98.9% (nucleotide) and 81.5-99.1% (amino acid) identitywith globally available BYMV sequences. Phylogeneticanalysis clustered them specifically in BYMV phylogeneticgroup-IV within the existing nine groups. The CK-GL1,CK-GL2, CK-GL4 and CK-GL5 isolates formed a discretecluster within group-IV. The present study suggests subdivisionof group-IV into subgroup-IVa and IVb. Moreover,infectivity assays using in vitro RNA transcripts fromsubgroup-IVa (CK-GL3 isolate) and IVb (CK-GL1 isolate)showed distinct biological differences between the isolatessupporting subdivision