Intergeneric hybrids between Enarthrocarpus lyratus, a wild species, and crop brassicas

Attempts were made to produce intergeneric hybrids between Enarthrocarpus lyratus, a wild species, and several species of crop brassicas: B. campestris, B. nigra, B. oleracea, B. juncea, B. napus and B. Carinata. Hybrids using E. lyratus as female parent were realized by means of embryo rescue in four combinations-E. lyratus × B. campestris, E. lyratus × B. oleracea, E. lyratus × B. napus and E. lyratus × B. carinata. Reciprocal crosses showed strong pre-fertilization barriers and yielded no hybrids except in one combination-B. Juncea × E. Lyratus-in which a single hybrid could be realized. All of the hybrids were multiplied in vitro through the multiplication of axillary shoots. Morphological and cytological studies confirmed hybridity. All hybrids were completely pollen sterile except for E. lyratus × B. carinata, which showed 2% pollen fertility. Attempts to double the chromosome number through the in vitro application of colchicine to axillary meristems of F1 hybrids were successful in only one hybrid, E. lyratus × B. oleracea. Cytological studies of the hybrids indicated the presence of a partial homology between the genomes of E. lyratus and crop brassicas. Backcross progenies were raised from all of the five F1 hybrids to develop malesterile alloplasmic lines