Merrifield resin and 1,6-hexanediol diacrylate-crosslinked polystyrene resin for solid-phase peptide synthesis: a comparative study

Three short peptides were synthesized on Merrifield resin (PS–DVB) and on polystyrene crosslinked with 1,6-hexanediol diacrylate resin (PS–HDODA) to compare their efficiencies in solid-phase peptide synthesis (SPPS). A 2% crosslinked polymeric system having almost equal capacity was used in both cases. The peptides were synthesized using standard solid-phase methodology. In the case of the PS–HDODA resin, all the couplings were completed by the first coupling and the peptides were obtained in > 90% yield and > 95% purity. But in the case of the PS–DVB resin, most of the attachments require two to three couplings and the peptides were obtained in about a 65% yield