Determination of absorption of vitamin B<SUB>12</SUB> by a double isotope tracer technique

A new simple method for the study of vitamin B12 absorption involving the use of two tracers is described. Along with 58Co labeled vitamin B12 an unabsorbable marker in the form of 51Cr labeled chromic oxide is administered to the patients. The ratio of the activities of the two isotopes in the standard is compared with the ratio of the activies of these isotopes in an aliquot of a stool sample collected after 24 or 48 hours. The absorption of labeled vitamin B12 is estimated from the alteration in the ratio of absorbable and unabsorbable isotopes in the stool sample. The method compares well with other methods of estimating vitamin B12 absorption such as Schilling's test, total faecal collection and whole body counting