SCREENING AND IDENTIFICATION ENDOPHYTIC BACTERIA FROM INDONESIAN BAY LEAVES (Eugenia polyantha WIGHT) WITH ANTIBACTERIA ACTIVITY

For many years, herbs already utilized as traditional medicine to cure diseases, particularly bacterial infections. One of potential herbs which reported has antibacterial activity is Eugenia polyantha Wight. This herbs is commonly used as spices for food by Indonesian people. Due to the significant increasing of multidrug resistance bacteria globally, the invention of new antibiotics is urgently needed. One of alternative ways finding active compounds to solve the problems is by using microbes inside plants. Unfortunately, no reports about endophytic bacteria from Indonesian Bay Leaves especially its potential as antibacterial. This research aimed to find endophytic bacteria isolates from Indonesian Bay Leaves which has antibacterial activity against Salmonella typhi. First, we isolated the bacteria from fresh E. polyantha leaves on agar medium then cultivated it using NB medium and continued to antibacterial assay by disc diffusion. The 16 sRNA of isolate with inhibition ability was sequenced. We got three endophytic bacteria isolates but only one isolate shown great antibacterial activity. According to the similarity result by BLAST analysis the isolate had similarity to Bacillus sp, thus, we named the isolate with B. subtilis strain WL-SM1