Utilization of Bromelain Enzyme from Pineapple Peel Waste on Mouthwash Formula Against Streptococcus mutans

The aim of this research was to apply bromelain enzyme from rough extract of Ananas comosus (L.) Merr. peel into the mouthwash preparation and investigate the enzyme activity to inhibit Streptococcus mutans growth.. The research method, bromelain was extracted by means of a blended pineapple skin, the extract was filtrated and centrifuged to obtain a supernatant. Enzyme activity was analyzed by spectrophotometer at 275 nm. Mouthwash formula which involved different concentration of bromelain (20%, 25%, 30%, 35%, 40%, 50%, and 60% (v/v)) was tested the antimicrobial activity by disc diffusion method. The results showed that the formula with 35% enzyme was an effective in restricting the growth of Streptococcus mutans, with a relative potential value of 100.40% compared to chlorhexidin 0.1% as possitive control