The development of high-speed rail (HSR) has had a notable impact on modal market shares on the routes on which its services have been implemented. The aim of this study is to analyse whether the HSR expansion in Italy has led to a modal shift from motorway to HSR. We empirically test i) whether HSR openings adjacent to motorway sectors have reduced the total km travelled by light vehicles on these sectors during the period 2001-2017; and ii) whether this reduction has been persistent or even more evident after the opening of on-track competition between two HSR operators. To do so, we carried out a generalized difference-in-differences estimation, using a unique panel dataset that exploits the heterogeneous traffic data within all tolled m...