The Bacillus pumilus 3-19 Glutamyl peptidase (EC 3.4.21.19) was isolated from the culture medium of the B. subtilis recombinant strain at the following stages of the bacillus growth: a decelerating growth phase and a stationary growth phase. The action of the purified preparations of the enzyme on different phases of its growth was studied on the insulin B-chain and various protein and peptide substrates. Physicochemical properties of the enzyme were compared for different phases of its growth. The glutamyl endopeptidase preparations differed in their catalytic characteristics and their sensitivity to the metal cations. © 2013 Pleiades Publishing, Ltd
The effect of nutrients and growth conditions on the accumulation of glutamyl endopeptidase in the c...
The effect of nutrients and growth conditions on the accumulation of glutamyl endopeptidase in the c...
The growth of the recombinant Bacillus subtilis strain AJ73 carrying the Bacillus intermedius 3-19 g...
The Bacillus pumilus 3-19 Glutamyl peptidase (EC 3.4.21.19) was isolated from the culture medium of ...
The recombinant strain of Bacillus subtilis bearing B. intermedius glutamyl endopeptidase gene in mu...
We studied the biosynthesis of bacillus intermedius glutamyl endopeptidase in the recombinant bacill...
The culture filtrate of Bacillus intermedius 3-19 was used for isolation by chromatography on CM-cel...
The culture broth of Bacillus amyloliquefaciens H2 was used for isolation by chromatography on CM-ce...
The biosynthesis of glutamyl endopeptidase from Bacillus intermedius 3-19 in recombinant strain of B...
The effect of nutrients and growth conditions on the accumulation of glutamyl endopeptidase in the c...
The effect of nutrients and growth conditions on the accumulation of glutamyl endopeptidase in the c...
The growth of the recombinant Bacillus subtilis strain AJ73 carrying the Bacillus intermedius 3-19 g...
The Bacillus pumilus 3-19 Glutamyl peptidase (EC 3.4.21.19) was isolated from the culture medium of ...
The recombinant strain of Bacillus subtilis bearing B. intermedius glutamyl endopeptidase gene in mu...
We studied the biosynthesis of bacillus intermedius glutamyl endopeptidase in the recombinant bacill...
The culture filtrate of Bacillus intermedius 3-19 was used for isolation by chromatography on CM-cel...
The culture broth of Bacillus amyloliquefaciens H2 was used for isolation by chromatography on CM-ce...
The biosynthesis of glutamyl endopeptidase from Bacillus intermedius 3-19 in recombinant strain of B...
The effect of nutrients and growth conditions on the accumulation of glutamyl endopeptidase in the c...
The effect of nutrients and growth conditions on the accumulation of glutamyl endopeptidase in the c...
The growth of the recombinant Bacillus subtilis strain AJ73 carrying the Bacillus intermedius 3-19 g...