Construction and characterization of the Bacillus strain with the inactivated phytase gene

The Bacillus subtilis dphy strain with the inactivated phytase gene was obtained. The phytase genes in bacilli displayed high homology; the respective enzymes belonged to the class of β-propeller phosphatases. Physiological and morphological features of the recombinant strain were determined and its sporulation was studied. The level of biomass accumulation was characterized under different conditions of bacterial growth: at different values of pH, temperature, and medium salinity. It is concluded that phytases may participate in stress response formation. © 2013 Pleiades Publishing, Ltd