Analysis of fibrogenesis indicators in patients with chronic hepatitis C

Робота присвячена вивченню сироваткових маркерів фіброзу печінки - трансформуючого фактора росту - βi (TGF-β1) і тканинного інгібітора металопротеінази-1 (ТІМП-1), та фіброеластометрії для встановлення ступеня фіброзу у хворих на хронічний гепатит С. Аналізуючи рівні цитокінів фіброгенезу та показники фіброеластометрії у 177 хворих на ХГС встановлено статистично достовірний зв’язок (р 6x105 lU/ml, respectively. Results and discussion. According to indirect transient elastometry in 14 (7,9%) CHC patients fibrosis was not revealed, in 22 (12.40%) the first stage was identified, in 38 (21.5%) - the second stage, in 82 (46.3 %) - the third stage and in 21 (11.9%) - the fourth stage of fibrosis according to METAVIR scale. While examining the levels of TGF-β1 and TIMPs-1 in patients with CHC it was determined that the level of TGF- β1 was elevated in 141 (79.7 %) of patients, reduced in 15 (8.5 %) and that corresponded to the normal values in 21 (11.9 %) of patients. The level TIMPs-1 was elevated in 130 (73.4 %) of patients, decreased in 12 (6.8 %) and that corresponded to indicators of control group in 35 (19.8 %). When studying the possible correlations between the levels of fibrogenesis cytokines and viral load it was determined that the level TGF- β1 and TIMPs-1 in CHC patients with high viral load was significantly higher (p<0.05) in comparison with the corresponding indicator at low viral load. Comparing the levels of cytokines and degrees of liver fibrosis according to indirect transient elastometry data it was stated the statistically significant correlation (p<0.01) between TGF-β1 and TIMPs-1 levels and the degree of liver fibrosis (r=0.78 and r=0.71) in CHC patients when liver fibrosis was F2 and higher. When examining relations between cytokines levels and concentration of cytolytic enzymes, the correlation between TGF-βi and TIMPs-1 concentration, on the one hand, and AST/ALT ratio, on the other (r=0.61, r=0.58, p<0.01 and r=0.68, r=0.62, p<0.01, correspondingly) was determined. Conclusions 1. In CHC patients the serum levels TGF-β1 and TIMPs-1 are significantly higher in case of the higher viral load than in low one. 2. Statistically significant correlation (p