CD8+ T-Cells Regulate Innate Immunity Decreasing Survival Post-Mi

Myocardial infarction (MI) is the most prevalent cause of death in the United States, indicating that the study of more effective post-MI treatment remains pertinent. In a recent study by DeLeon-Pennell et al., it was determined that CD8+ T-cells regulate innate immune response, thereby increasing 7-day survival rates post-MI. The main objective of this study was to further explore how the post-MI environment would regulate CD8+ T-cell physiology. The T-cells were cultured in 0.10% FBS (unstimulated), IL4, IL12, IL4+MMP9, and IL12+MMP9 for 4 hours, and conditioned media were collected and analyzed using a mass spectrometer. Clustering of the dataset using Pearson\u27s coefficient and Markov\u27s Clustering algorithm showed five main clusters corresponding to the five main groups. A one-way Analysis of Variance (ANOVA) and volcano plot was created to determine proteins of statistical significance in the 4-hour treatment. Actin, antithrombin-III, amyloid-beta A4, collagen alpha-5(IV) chain, exocyst complex component 4, and GMP reductase 2 were among a few statistically significant proteins in the 4-hour treatment as determined by ANOVA. Additionally, we found that certain protein secretions were correlated by gender. In conclusion, our study suggests that the post-MI environment is facilitating in CD8+ T-cell function and thus facilitating the post-MI wound healing progression