Add to ORKGMultiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have facilitated powerful biological engineering applications, vastly enhancing the scope and efficiencies of genetic editing and transcriptional regulation. In this review, we discuss multiplexed CRISPR technologies and describe methods for the assembly, expression and processing of synthetic guide RNA arrays in vivo. Applications that benefit from multiplexed CRISPR technologies, including cellular recorders, genetic circuits, biosensors, combinatorial genetic perturbations, large-scale genome engineering and the rewiring of metabolic pathways, are highlighted. We also offer a glimpse of emerging challenges and emphasize experimental considerati...
With rapid progress in DNA synthesis and sequencing, strain engineering starts to be the rate-limiti...
From its discovery as an adaptive bacterial and archaea immune system, the clustered regularly inter...
Engineered DNA-binding proteins that manipulate the human genome and transcriptome have en-abled rap...
Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have ...
Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have ...
The ability to modify multiple genetic elements simultaneously would help to elucidate and control t...
The ability to modify multiple genetic elements simultaneously would help to elucidate and control t...
The ability to modify multiple genetic elements simultaneously would help to elucidate and control t...
The ability to modify multiple genetic elements simultaneously would help to elucidate and control t...
The CRISPR/Cas9 system is an RNA guided nuclease system that evolved as a mechanism of adaptive immu...
The CRISPR/Cas9 system is an RNA guided nuclease system that evolved as a mechanism of adaptive immu...
CRISPR-based genome editing provides a simple and scalable toolbox for a variety of therapeutic and ...
SummaryTargeted gene regulation on a genome-wide scale is a powerful strategy for interrogating, per...
A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libra...
RNA-based regulation and CRISPR/Cas transcription factors (CRISPR-TFs) have the potential to be inte...
With rapid progress in DNA synthesis and sequencing, strain engineering starts to be the rate-limiti...
From its discovery as an adaptive bacterial and archaea immune system, the clustered regularly inter...
Engineered DNA-binding proteins that manipulate the human genome and transcriptome have en-abled rap...
Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have ...
Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have ...
The ability to modify multiple genetic elements simultaneously would help to elucidate and control t...
The ability to modify multiple genetic elements simultaneously would help to elucidate and control t...
The ability to modify multiple genetic elements simultaneously would help to elucidate and control t...
The ability to modify multiple genetic elements simultaneously would help to elucidate and control t...
The CRISPR/Cas9 system is an RNA guided nuclease system that evolved as a mechanism of adaptive immu...
The CRISPR/Cas9 system is an RNA guided nuclease system that evolved as a mechanism of adaptive immu...
CRISPR-based genome editing provides a simple and scalable toolbox for a variety of therapeutic and ...
SummaryTargeted gene regulation on a genome-wide scale is a powerful strategy for interrogating, per...
A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libra...
RNA-based regulation and CRISPR/Cas transcription factors (CRISPR-TFs) have the potential to be inte...
With rapid progress in DNA synthesis and sequencing, strain engineering starts to be the rate-limiti...
From its discovery as an adaptive bacterial and archaea immune system, the clustered regularly inter...
Engineered DNA-binding proteins that manipulate the human genome and transcriptome have en-abled rap...