Influence of secretions from individual salivary glands on bacterial adsorption to hydroxyapatite surfaces

PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.Thesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Graduate Dentistry (Oral Microbiology), 1981.Bibliography: leaves 63-71.The adsorption of Streptococcus mutants, strains H12 and W1-10, Actinomyces naeslundii,strain 55N Actinomyces viscosus,strain CK8, Capnocytophaga orchraceus, strain 25, Capnocytophaga gingivalis,strain 27, to hydroxyapatite coated with samples of parotid, submaxillary, or whole saliva was studied by use of a radioactive assay, using 3H-labelled bacterial cells at a concentration of 5 x 10 superscript 7 cells/ml, and 10 mg hydroxyapatite (HA) beads. Adsorption to untreated and saliva-treated HA was compared. Whole saliva and glandular secretions were collected from five subjects. It is found that a greater number of cells adsorbed to uncoated HA than to HA coated with whole or parotid saliva, for all strains studied except Actinomyces viscosus CK8. The sorption of the strains studied to HA treated with submaxillary saliva was in all but one instance greatly increased as compared to the other salivary coatings, and comparable to the sorption to the uncoated HA. Compared to parotid saliva whole sativa sometimes increased the number of S. mutans W1-10 and A. naeslundii, strain 55N cells which attached, and often reduced the number of the other strains which adsorbed. Species differences in adherence were also observed. The adherence of A. viscosus was significantly greater than that of the other bacteria. Capnocytophaga gingivalis and A.naeslundii showed the lowest affinity for HA. S. mutants and Capnocytophaga ochraceus adhered comparably. The data obtained suggest that the salivary pellicles which form on teeth may differ depending upon their proximity to individual salivary glands in the mouth. These differences may influence the types of bacteria which subsequently attach to the teeth